Part 1: pGLO Bacterial Transformation and DNA Extraction June 27th, 2017
Transformation: Uptake and expression of a gene from one organism to another
Creates a transgenic organism with recombinant DNA
Cutting and pasting DNA from source to new cells
- Cutting: Restriction enzymes
- Pasting: Ligases
In this experiment
Recipient: E. Coli bacterial cells
Vector: pGLO plasmid
- Genes of interest:
- GFP gene= green fluorescent protein
- bla gene= resistant to ampicillin
- araC gene= allows GFP to be expressed
Standardized variables: LB infused agar
Conclusions: The +DNA LB/ Amp was able to successfully grow colonies, but notice that they don’t glow. The treatment group that was +DNA LB/ AMP/ ARA was able to use the gene for fluorescence with the presence of arabinose sugar.
Part 2: Polymerase Chain Reaction (PCR) June 29th, 2017
Based on DNA replication
Used in forensic science, detect mutations, paternity testing
Process details:
- Denature DNA to break it apart into two strands (95 C)
- Anneal primers to bind to target
- Extend primers to taq polymerase enzyme
Part 3: Gel Electrophoresis July 6th, 2017
Method of sorting macromolecules (especially proteins and nucleic acids) based on their size and charge
Without UV Light With UV Light
No comments:
Post a Comment