Biochemical Analysis May 23rd, 2017
Use chemical reagents to test for the presence of several common organic compounds
Objectives:
Part 1: To identify a positive reaction for six chemical reagents commonly used to test for the presence of various organic compounds.
Part 2: To use your skills from part 1 to investigate the chemical composition of a solution of an unknown chemical composition.
Starch “Iodine” Test: Sugar “Benedict’s” Test:
Protein “Biuret” Test: Amino Acid “Ninhydrin” Test:
Part 1: Identify Known Sample for Common Organic Compounds
Test
|
Amount of Substance
|
Amount of Reagent
|
Positive Result
|
Negative Result
|
Observations
|
Starch
|
2 mL
|
2 drops IKI
|
Blue/ Black
|
Yellow
|
Precipitate forms
|
Sugar
|
2 mL
|
1 mL Benedict
|
Red/ Brown
|
Bright Blue
| |
Protein
|
2 mL
|
Drop by drop
Biuret
|
Lavender
|
Light Blue
|
Goes away with time
|
Amino Acid
|
2 drops
|
Douse with Ninhydrin
|
Purple
|
White
|
Be careful of contaminates
|
Lipid
|
2 drops
|
Douse with Acetone
|
Oil
|
No Oil
| |
Vitamin C
|
25 mL
| 6 mL Iodine |
Slight purple
|
No Change
|
Part 2: Identify Unknown Sample for Common Organic Compounds
Starch
|
Sugar
|
Protein
|
Amino Acid
| |
Unknown A
| + | - | - | - |
Unknown B
| + | + | + | - |
Unknown C
| + | - | + | - |
Unknown D
| - | + | - | + |
No comments:
Post a Comment